HLA I and HLA II – Class I and Class II histocompatibility antigens
HLA = Human Leukocyte Antigens – these antigens give identity to a person’s tissues. In order for the transplant result to be as good as possible, it is necessary to establish a maximum similarity (match) between donor and recipient. Genotyping of the HLA genes establishes the match (the match between the HLA antigens of the donor and the recipient) with maximum precision.
HLA typing
- PCR – SSP
- Luminex
- PCR – SBT
- NGS (Next Generation Sequencing)
Chimerism
LUMINEX cytotoxic antibodies
- screening – detects the presence in the recipient of antibodies against the donor’s HLA antigens.
- identification – identifies the specificities of previously detected anti-HLA antibodies.
Crossmatch: LUMINEX
- Crossmatch through LUMINEX – using the lymphocytes collected from the potential donor and the serum from the recipient, the possible presence of antibodies against HLA class I and class II antigens of the donor is detected at the recipient. A positive crossmatch generally indicates a contraindication for transplantation.
Disease associations
- HLA B27
- DQ 2
- DQ 8
- Quantitative dosing of Tacrolimus (Prograf) in total blood by the MEIA method.
- Quantitative dosage in total blood of Ciclosporin (Neoral) by the MEIA method.
- Quantitative dosing of Sirolimus (Rapamune) in total blood by the MEIA method.
- Quantitative dosing of Methotrexate in total blood by the CMIA method.
Immunosuppression – the monitoring of the immunosuppressive therapy is performed through i2000SR ABBOTT, post transplant, in order to quantify the serum level of the administered immunosuppressant and establish the subsequent doses.
- AFP – Alpha Feto-Protein
- CEA – Carcino-Embryonic Antigen
- CA 19-9 – Cancer Antigen 19-9
- CA 125 – Cancer Antigen 125
- CA 15-3 – Cancer Antigen 15-3
- PSA Free – Prostate Specific Antigen – the free fraction
- PSA Total – Prostate Specific Antigen – total
All these tumor markers are useful in assessing the patient’s status both pre-operatively and post-operatively.
- IL1? – Interleukin 1 beta
- IL6 – Interleukin 6
